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File formats

Immunology pipelines accept tabular CSV/TSV/JSON inputs where possible. Many endpoints also accept inline row arrays.

Immune composition

Recommended columns:

ColumnPurpose
cell_typeImmune population label
groupOptional group, condition, or sample

Annotation

Recommended columns:

ColumnPurpose
cell_idCell identifier
broad_cell_typeBroad immune label
gene columnsOptional expression values for gene program scoring
groupOptional grouping

Repertoire

Supported conventions:

ConventionExample fields
10x VDJbarcode, chain, cdr3, v_gene, j_gene, raw_clonotype_id
AIRRlocus, junction_aa, v_call, j_call, duplicate_count
Bulk TCR/BCRsample_id, clonotype_id, count

Communication

Expression rows should contain cell IDs and gene columns. Metadata rows should contain the same cell IDs and a cell type column.

Disease workflows

Useful columns:

  • cell_id
  • sample_id
  • cell_type
  • immune_state
  • clonotype_id
  • pseudotime
  • x, y
  • cytokine_score
  • disease_label
  • disease_activity_score
  • medication
  • timepoint
  • vaccination_infection_history

Multi-modal profiling

ModalityColumns
ADTcell_id plus protein columns
ATACpeak_id, gene, cell_id, accessibility
Cytometry eventsevent_id, sample_id, marker columns such as CD3, CD19, CD14, CD56

Binary FCS is feature-gated until a FlowUtils-compatible reader is configured.