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Cytokine signaling

Use this workflow to summarize immune signaling between cell populations.

Research questions

  • Which sender/receiver pairs have strong ligand-receptor scores?
  • Which cytokine pathways are prominent?
  • Which signaling pathways differ by disease, treatment, or response group?

Suggested path

  1. Register expression and metadata inputs.
  2. Run cell_communication.
  3. Review signaling bubbles, communication matrix, pathway heatmap table, and comparative deltas.
  4. Generate interpretation from completed communication runs.

Outputs to cite

  • interaction score
  • pathway score
  • ligand activity score
  • comparative delta
  • p-value when permutations are requested